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Total RNA-Seq

The transcriptome is the total set of transcripts, including mRNA and non-coding RNA (e.g. small RNAs and microRNAs), in a population of cells under specific conditions. Whole-transcriptome sequencing involves the combined application of mRNA-Seq and small RNA-Seq to capture both coding and non-coding regions.

Platform Illumina MiSeq, Ion Proton, Ion PGM™
Deliverables
  • Analytical report
  • Raw FASTQ files
Research Objectives This application can be used for de-novo transcript assembly, identification of genes with low levels of expression, isoform detection, quantification of differential expression in different organisms or different tissues in the same organism, SNP discovery, genotyping, RNA editing, and allele-specific expression.
Related Services
  • Exome Sequencing
  • Axiom Plate Array

Overview

The transcriptome is the total set of transcripts, including mRNA and non-coding RNA (e.g. small RNAs and microRNAs), in a population of cells under specific conditions. Whole-transcriptome sequencing involves the combined application of mRNA-Seq and small RNA-Seq to capture both coding and non-coding regions. This application can be used for de-novo transcript assembly, to identify genes with low levels of expression, to detect isoforms, to quantify differential expression in different organisms or different tissues in the same organism, SNP discovery, genotyping, RNA editing, allele-specific expression.

Service Details

CPGR will accept purified RNA samples that conform to the minimum requirements. RNA samples will undergo QC using the Bioanalyser. Library preparation and construction will be performed according to project specific requirements followed by high throughput sequencing. The raw fastQ files are used as an input for standard bioinformatics analysis.

Service Deliverables

The client will in the first instance receive a sample QC report detailing whether the integrity of the sample meets the requirements for the application. Following sequencing the client will receive an analytical report detailing the parameters of as well as the results of the sequencing run including the raw fastQ files which can be used as the input for standard bioinformatics.

Starting Material

  • RNA Quantity
    ≥ 5µg (prokaryotic, human, rat and mouse)
    ≥ 10µg (non-model animals)
    ≥ 20µg (plants and fungal)
  • RNA Concentration:
    60 ng/µl (prokaryotic, human, rat and mouse)
    150 ng/µl (non-model animals)
    250 ng/µl (plants and fungal)