|Platform||Illumina MiSeq, Ion Proton, Ion PGM™|
|Research Objectives||Examination of tissue specific expression patterns, disease associations, isoforms of miRNAs, and discovery of novel miRNAs.|
miRNA-seq input material is often enriched for small RNAs. miRNA-seq allows researchers to examine tissue specific expression patterns, disease associations, isoforms of miRNAs, and to discover novel miRNAs.
CPGR will accept purified RNA samples that conform to the minimum requirements. RNA samples will undergo QC using the Bioanalyser before miRNA enrichment, and library preparation and construction according to project specific requirements. This will be followed by high throughput sequencing. The raw FASTQ files are used as an input for standard bioinformatics analysis.
The client will in the first instance receive a sample QC report detailing whether the integrity of the sample meets the requirements for the application. Following sequencing the client will receive an analytical report detailing the parameters and the results of the sequencing run, including the raw FASTQ files. These files can be used as the input for standard bioinformatics.
- RNA Quantity:
≥ 5 µg (prokaryotic, human, rat and mouse)
≥ 10 µg (non-model animals)
≥ 20 µg (plants and fungal)
- RNA Concentration:
60 ng/µl (prokaryotic, human, rat and mouse)
150 ng/µl (non-model animals)
250 ng/µl (plants and fungal)