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Microbial Genome Sequencing

Microbial genome sequencing refers to sequencing the complete nucleotide sequence of a prokaryotic organism using high throughput Next Generation Sequencing (NGS) technologies. The genome sequence includes both coding and non-coding regions of DNA. The service is available for all microbial organisms with or without the availability of a reference sequence.

Platform Illumina MiSeq, Ion Proton, Ion PGM™
Deliverables
  • Sample QC report
  • Analytical report (including sample and library preparation – mate pair, pair end or single end)
  • Raw FASTQ files
Research Objectives Resolve genetic diversity between the same or closely related species and identify novel or known sequence variations.
Related Services
  • Targeted resequencing
  • Prokaryotic Genome Annotation

Overview

Microbial genome sequencing enables researches to address genetic diversity between the same or closely related species. It also enables studies interested in cataloguing novel or known sequence variation. NGS technology is implemented to allow for more rapid and accurate data generation.

Service Details

CPGR will accept purified DNA samples that conform to the minimum requirements. DNA samples will undergo QC using the Quant-iT™ PicoGreen® dsDNA assay kit. Library preparation and construction will be performed according to project specific requirements followed by high throughput sequencing. The raw FASTA or FASTQ files are used as an input for standard bioinformatics analysis.

Service Deliverables

The client will in the first instance receive a sample QC report detailing whether the integrity of the sample meets the requirements for the application. Following sequencing the client will receive an analytical report detailing the parameters and the results of the sequencing run including the raw FASTA and/or FASTQ files which can be used as the input for standard bioinformatics applications.

Starting Material

  • DNA Quantity: ≥ 6 µg
  • DNA Concentration: 60 ng/µl