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Eukaryotic Genome Sequencing

Eukaryotic Genome sequencing refers to sequencing the complete nucleotide sequence of a eukaryotic organism using high throughput Next Generation Sequencing (NGS) technologies. The genome sequence includes both chromosomal DNA (genes and non-coding regions) as well as DNA contained in mitochondria (or chloroplasts in the case of plants). The service is available for all eukaryotic organisms with or without the availability of a reference sequence.

Platform Illumina HiSeq 2000/2500
Deliverables
  • Sample QC report
  • Analytical report (including sample and library preparation – mate pair, pair end or single end)
  • Raw FASTQ files
Research Objectives Genetic diversity studies between the same or closely related species and studies interested in cataloguing sequence variation.
Related Services
  • Exome Sequencing
  • Genome-wide Human SNP 6.0 Array
  • Axiom Plate Array

Overview

Eukaryotic genome sequencing enables researches to address genetic diversity between the same or closely related species and is can be applied in studies cataloguing novel or known sequence variation associated with a particular condition. The advantage of the Illumina HiSeq is its high throughput capacity, capable of generating read lengths of 2 X 100 bp yielding approximately 600 Gb in a single run.

Service Details

The CPGR will accept purified DNA samples conform to minimum requirements. DNA samples will undergo QC analysis using the Quant-iT™ PicoGreen® dsDNA assay kit. Library preparation and construction will be performed according to project specific requirements followed by high throughput sequencing. The raw FastQ  files are used as an input for standard bioinformatics analysis.

Service Deliverables

The client will in the first instance receive a sample QC report detailing whether the integrity of the sample meets the requirements for the application. Following sequencing the client will receive an analytical report detailing the parameters and the results of the sequencing run, including the raw FASTQ files. These files can be used as the input for standard bioinformatics.

Starting Material

  • DNA Quantity: ≥ 6 µg
  • DNA Concentration: 60 ng/µl