DNA Concentration and Purity Determination
Measure extracted DNA sample concentration and purity
|Deliverables||Analytical report with DNA sample purity and concentration|
|Research Objectives||Assess the efficiency of the method of DNA extraction, DNA sample concentration and purity|
To ascertain the efficacy of the method of DNA extraction and assess DNA sample concentration and purity before use in downstream applications.
Samples are delivered to the CPGR on dry ice (RNA), at room temperature or on wet ice. An aliquot of the sample (1-2 µl) is removed and measured on the Nanodrop ND 1000 after blanking with DNAse and RNAse Free Water or eluent buffer provided with the DNA extraction kit. If the presence of contaminants, salts and/or inhibitors is suspected (260/230 ratios >1.5), ethanol precipitation will be performed to clean up the samples.
An analytical report will be provided detailing sample concentration and purity. If sample clean up (ethanol precipitation) is required, the steps taken will be included in the report. The final DNA sample will be returned to the client when and if necessary.
- DNA Concentration: 2 – 3700 ng/µl