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Proteomics


Mass spectrometry
PMF Peptide Mass Fingerprinting (PMF) from samples digested either in solution or in gel slices. PMF confirmation can be provided via MS/MS analysis; Details...
Intact mass Intact protein masses can be determined for proteins and large peptides in the range m/z = 4 000 -200 000. A greater dynamic range is possible if the protein forms multiple charges;
LC-MS Mudpit analysis based on either 1D or 2D nano-LC (Dionex Ultimate 3000) followed by MS/MS analysis. Up to 1664 fractions can be collected and spotted on a MALDI source plate by a robotic spotter (LCPackings Probot); Details...
iTRAQ Isobaric isotope labels that can be attached to peptides prior to LC-MS allowing relative (or absolute if an internal standard is available) quantitation of up to 8 protein samples; ideally suited for protein biomarker discovery; Details...
Data analysis In addition to the Mascot search algorithm data can also be analysed with the advanced ProteinPilot (Applied Biosystems International) Paragon algorithm. This algorithm provides high confidence analysis with an unprecedented 150 variable modifications;
Protein QC/QA Quality control analysis for protein and peptide products can be developed to customers specifications.


Protein microarrays
Peptide arrays Low- to intermediate density sandwich immunoassays on various protein array substrates; Details...
Extract arrays Printing of crude or partially purified biological extracts for analysis of biological activity (e.g. antibody binding to immunogenic proteins); Details...
Protein arrays Arrays of purified single proteins in recombinant or natural form for protein-protein, protein-antibody or protein-molecule interaction; Details...
Biomarker Discovery Arrays Cancer Antigen (CT-100) Biomarker discovery arrays Details...
Protein array printing Custom printing of a variety of protein array formats on a standard high-throughput QArray2 (Genetix) contact printer
Microarray hybridization Standard protein hybridizations done in an automated processing and incubation station (Tecan HS 4800) in single, dual or quad format or in flexible 1-16 array-per slide formats (automated assays done in a Tecan HydroFlex);
Scanning All scanning done in 4u microarray reader compatible with most existing microarray designs and formats as well as with arrays printed in 96 MTP formats;
Compatibility All Cy3 or Cy5 labelled analytes and detection antibodies; colorimetric assays and read-out also available.


BioPlex
Antibody assays Multiplex bead-based sandwich immunoassays (typically 25 data-points per assay), e.g. for cytokine profiling Details...
Compatibility Linco, BioRad, Luminex


Bioanalyzer
Protein Chip Protein size, integrity and concentration (5-250 kD) Details...
Sample Prep Genomics Proteomics Bioinformatics Cell Culture Recombinant Protein Forms
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