Service Title
Taqman® TM Gene Expression
Workflow Code
PCR-TAQ
Short Service Description
Analyse gene regulation / expression in a quantitative fashion using customised or inventoried gene expression panels.
Base Price (Price per unit)
Price on enquiry
Turn Around Time
1 working day day (per 768 reactions post delivery of samples and reagents)
Detailed Description and background
TaqMan® Gene Expression Assays consist of a pair of unlabelled PCR primers and a TaqMan® probe with a FAM™ or VIC® dye label and minor groove binder (MGB) moiety on the 5' end, and non-fluorescent quencher (NFQ) dye on the 3' end. RNA from samples of interest is reverse transcribed into cDNA, and the synthesized cDNA serves as the template for real-time PCR.
Service Details
Taqman® assays are purchased singularly for each gene under investigation. Each assay is able to perform up to 250 reactions. 19 species have been represented in over 1,200,000 pre-designed TaqMan® Assays. Customized assays can be developed to study any gene or splice variant in any organism. The workflow includes a reverse transcription step whereby the ABI High Capacity RNA-to-cDNA Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range. This is followed by the master mix preparation designed to exceed the performance of standard master mixes,(<40 minutes) and finally real-time quantitative PCR on the 7900HT utilizing the 384-well plate format and offering optional fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System at the CPGR combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.
Service Deliverable
Report includes the QC quality gate results, the raw data and an analysis report. Bioinformatic analysis and interpretation of data is optional.
Sample/Info Submission Info
From 1 to 384 reactions may be processed in a single run on each 384 well plate. Each assay kit is able to perform up to 250 reactions and each sample is run in triplicate or quadruplecate together with a standard curve, (if not persuing the Δ Δ CT quantitative method). The concentrations of RNA per sample required is a minimum of 150ng in 10uL for effective cDNA synthesis for 80-100 reactions. RNA must be proven good quality(bioanalyzer trace) and 260/280 and 260/230 ratios between 1.8 and 2.1.
Pricing Details
Price on request.
Key Words
gene expression. Taqman assay. qPCR. RT-PCR. Genomic DNA. Pathway analysis. RNA. cDNA .
Sample Shipping Address
Institute of Infectious Disease and Molecular Medicine, UCT, Faculty of Health Sciences, Wernher and Beit Building, Level 2, Lab S2.09, Anzio Road, Observatory, Cape Town 7925, South Africa.
Related services
Bioinformatics. Sample Preparation.
Printable Version
A downloadable PDF Print Version of this service can be found here.
Target Gene Expression
