Service Title
Peptide Mass Fingerprinting
Workflow Code
MASS_PMF
Short Service Description
Database dependent identification of purified proteins
Base Price (Price per unit)
Price on enquiry
Turn Around Time
5 days
Detailed Description and Background
Peptide Mass Finger printing relies on matching the unique peptide distribution of a protein against peptide patterns of known proteins in a protein database. A the CPGR a control protein is included to verify that complete digestion did occur as well as a gel blank digest for gel separated proteins. The controlled, validated workflow guarantees that reliable data are reported every time
Service Details
In-gel or in-solution samples are subjected to trypsin digest. The resultant peptides are cleaned up before spotting onto a MALDI source plate. MS spectra are acquired for database interrogation and where possible results confirmed using MS/MS
Service Deliverable
Report includes database search results as well as control data for the experiments
Sample/Info Submission Info
Up to 20 samples can be submitted as desiccated gel slices from either Coomasie of MS compatible silver stain gels per batch. Alternatively pure samples may be submitted for in-solution digest
Pricing Details
Price on request
Key Words
Peptide Mass Fingerprinting, Protein Identification, MALDI, MS/MS Biomarker discovery
Sample Shipping Address
Institute of Infectious Disease and Molecular Medicine, UCT, Faculty of Health Sciences, Wernher and Beit Building, Level 2, Lab S2.09, Anzio Road, Observatory, Cape Town 7925, South Africa
Related services
Bioinformatics, protein arrays, protein expression, Luminex
Printable Version
A downloadable PDF Print Version of this service can be found here.
Protein Identification
