High-density DNA
microarrays can be used to unravel how entire regions of chromosomes have been modified or
damaged, providing unprecedented levels of sensitivity and complexity and leading to a better understanding how DNA copy number correlates with the development of complex diseases.High-throughput microarray
and RT-PCR applications can also be used to study genetic variations at the level
of individual genes or single nucleotide polymorphisms (SNPs), to address gene-gene or gene-disease interactions on a global scale, or to search for diagnostic markers and novel drug targets. The
CPGR also provides access to a range of next-generation sequencing technologies through partner laboratories in order to support for genome-scale de-novo sequencing or re-sequencing efforts. The CPGR
will assist with study design, sample preparation, target DNA enrichment (sequence capturing) and data-analysis. The complex machinery of transcriptional regulation can be explored using
state-of-the-art DNA microarrays that permit the study of mRNA expression at the level of individual genes, revealing the up- and down-regulation of transcription in clinical or plant samples. In
addition, new cutting-edge novel microarray technology can be used to examine the expression of every individual exon in the human genome in order to reveal previously unknown alternative splicing
events involved in cell regulation or disease development. In addition, the subtle regulation of gene expression in eukaryotic cells by small microRNA (miRNA) molecules can be studied using
high-density microarrays or sensitive RT-PCR assays.
In addition, the subtle 
regulation of gene expression in eukaryotic cells by small microRNA (miRNA) molecules
can be studied using high-density microarrays or sensitive RT-PCR assays. A greater level of detail can be obtained through complete RNA and miRNA sequencing using a suitable next-generation
sequencing platform. The CPGR offers support for study design, sample preparation and bioinformatic analysis of sequence data, employing a range of custom-built data-analysis pipelines. Contact the
CPGR to find out how an integrated panel of cutting-edge high-throughput biological applications can help you reach
